Rat S100A8 (Protein S100-A8) ELISA Kit
SKU: 89928810386

Rat S100A8 (Protein S100-A8) ELISA Kit

Sale price$234.00 Regular price$260.00
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Description

Rat S100A8 (Protein S100-A8) ELISA KitThe Rat S100A8 (Protein S100 A8) ELISA Kit measures S100A8 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti S100A8 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Rat S100A8 (Protein S100-A8) ELISA Kit measures S100A8 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti S100A8 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with S100A8 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of S100A8 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 89928810386

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VanillaPepper
Waukegan, US
★★★★★ 5
Surprisingly Durable, Very Soft, and All of My Dogs Immediately Loved It
Color: Green
I honestly expected this to be just another cute plush squeaky toy that would last maybe a day or two in a house with multiple dogs, especially larger breeds, but I was pleasantly surprised by how well this one has held up so far. The fabric is very soft without feeling overly thin or cheaply stuffed, and the toy arrived fully intact with no strange chemical smell right out of the packaging, which I always appreciate when buying toys for dogs that immediately put everything in their mouths. The stitching also looked much better than many inexpensive plush toys I’ve purchased in the past. All of my dogs immediately gravitated toward it — from my Dobermans to my smaller dogs — which honestly says a lot because they can be very selective about textures and squeakers. The squeaker itself is loud enough to keep their attention without being one of those painfully obnoxious high-pitched squeaks that make you regret purchasing the toy five minutes later. I also appreciate that the fabric is soft enough for indoor play and carrying around the house without feeling overly rough or stiff the way some “durable” dog toys can feel. It has held up surprisingly well to daily tossing, carrying, squeaking, and general chaos so far. For the price, I actually think this is a very good value, especially if you have multiple dogs or dogs that enjoy plush comfort toys but still like squeakers and interactive play. It arrived quickly, packaged well, and looked exactly like the listing photos upon arrival. Very happy overall and would absolutely purchase additional styles again. #FoundItOnAmazon #AmazonFinds #DogMom #DobermanMom #DogToys #PetSupplies #DogLife #LargeBreedDogs #SmallDogs #DogLovers #PetLifestyle #EverydayFavorites #LuxeLife
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Reviewed in the United States on May 11, 2026
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Verified Purchase
Junio
Draper, US
★★★★★ 5
Good quality. Excellent price
Color: Green
Very durable and lots of fun!
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Reviewed in the United States on May 4, 2026
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Myrna S
Los Angeles, US
★★★★★ 4
Fun toy but not as durable as I hoped.
Color: Green
I just rescued a dog from the shelter. She is super sweet and playful but she loves to chew her toys so I needed to buy her ones more durable to play with. This toy caught my eye but I was concerned that it might be too large for my small 7 pound terrier mix. After receiving it, I really loved the colors and the way it looked and my dog loves playing with it. Everything about it is great except that within a few weeks of playing with it, she already managed to make a small tear in it. It's not to the point where I would take it away from her toy box but I just wish it was more durable.
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Reviewed in the United States on November 8, 2025
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Amazon Customer
Omaha, US
★★★★★ 5
Great for tug of war
Color: Green
All the arms make for a good tug of war toy. It’s tough enough that my 70 lbs lab can pull on it and it’s fine
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Reviewed in the United States on May 30, 2026
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A. Dykes
Lexington, US
★★★★★ 5
Durable
Color: Green, Color: Green
We’ve had it for awhile now and it’s still (mostly) in one piece. Has held up well for our heavy chewing puppy. It lost a tentacle but that’s nothing compared to the level of destruction our dog has put other toys through in much shorter amounts of time. Recommend for heavy chewers and will likely buy again!
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Reviewed in the United States on February 24, 2026

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