Human 3MH (3-methylhistidine) ELISA Kit
SKU: 87027427025

Human 3MH (3-methylhistidine) ELISA Kit

Sale price$234.00 Regular price$260.00
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Description

Human 3MH (3-methylhistidine) ELISA KitThe Human 3MH (3 methylhistidine) ELISA Kit measures 3MH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 3MH. 3MH in the sample or standard competes with a fixed amount of biotin labeled 3MH for sites on a pre coated antibody specific to 3MH. Free components are washed off. After adding

The Human 3MH (3-methylhistidine) ELISA Kit measures 3MH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 3MH. 3MH in the sample or standard competes with a fixed amount of biotin-labeled 3MH for sites on a pre-coated antibody specific to 3MH. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 3MH in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 87027427025

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Need scan button to work!
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Love (almost) everything about this except the scan button. The scan button on the Epson screen does not work. I called Microsoft who tried to fix it through the connection but still didn't work. Sent an e-mail to Epson and no response. Really need this scan button to work so that I can scan to desktop.
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