SKU: 52759578727

Human CLDN18 (Claudin-18) ELISA Kit

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Description

Human CLDN18 (Claudin-18) ELISA KitThe Human CLDN18 (Claudin 18) ELISA Kit measures CLDN18 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CLDN18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human CLDN18 (Claudin-18) ELISA Kit measures CLDN18 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CLDN18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CLDN18 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CLDN18 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 52759578727

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L
LibraryMom
Boise, US
★★★★★ 5
Love!
Format: Hardcover
A little girl feels like she can courageously tackle anything with the help of her brave lion. When she climbs a tall slide, however, and her lion becomes afraid, she must summon her own strength to overcome her fear.  I especially love the illustrations in this validating and reassuring read.
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Reviewed in the United States on October 5, 2023
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Kim B.
Fort Morgan, US
★★★★★ 5
Wonderful Picture Book!
Format: Hardcover
During tonight's bedtime reading, the captivating illustrations had my toddler completely engrossed, eagerly demanding, "again, again!" The blend of fun and valuable lessons in the story is truly a standout. It brilliantly addresses concepts of fear and doubt, offering young readers a guide on how to conquer them.
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Reviewed in the United States on August 30, 2023
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Laura Harrison
Charlottesville, US
★★★★★ 5
Everything a Picture Book Should Be!
Format: Hardcover
Such a great picture book. Fun, vibrant illustrations. It is so nice that As Brave as a Lion is also available in Spanish. A terrific storytime, bedtime and classroom read. The book is simple but with a great message. Highly recommend.
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Reviewed in the United States on August 12, 2023
Y
Young Family
San Leandro, US
★★★★★ 5
Love it
Format: Hardcover, Format: Hardcover
As Brave As A Lion is a needed, heartwarming, precious, whimsical, lesson-filled, and fun book! This wonderful story highlights fear, bravery, and risk taking. It is the perfect story to teach children the challenges of bravery and the reward of bravery. Sometimes someone helps us find our courage and other times we have to help them find theirs. In this story the young girl feels lots of emotions and faces several challenges. However, she knows that her lion is always there with her and draws on his strength. He is her best friend. One day a new super fast slide is put in at the playground. She is so excited to go on the slide.....until she reaches the top. When she turns to her lion, she sees he is scared too. Will they ever find the courage to go down the slide?
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Reviewed in the United States on November 9, 2023
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CB
Whiting, US
★★★★★ 5
got it
Format: Hardcover
got it when i was supposed to but can't review it anymore as it was a gift. it would be nice to just be able to star something & not HAVE TO write 20 words.
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Reviewed in the United States on May 30, 2013

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