Human Anti- ITIH4 (Inter-alpha-trypsin inhibitor heavy chain H4) antibody ELISA Kit

Human Anti- ITIH4 (Inter-alpha-trypsin inhibitor heavy chain H4) antibody ELISA KitThe Human Anti ITIH4 (Inter alpha trypsin inhibitor heavy chain H4) antibody ELISA Kit measures Anti ITIH4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively.

The Human Anti- ITIH4 (Inter-alpha-trypsin inhibitor heavy chain H4) antibody ELISA Kit measures Anti- ITIH4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Anti- ITIH4 bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti- ITIH4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.