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Description
human pgp vesicle kit hek293P Glykoproteine MDR1 Assay 100 Reaktionen Das Human Pgp Vesicle Kit enthlt Inside out Membranvesikel aus HEK293 Zellen, die transient P Glykoprotein (P gp MDR1 ABCB1) berexprimieren. P gp ist einer der klinisch relevantesten Efflux Transporter und ist an zentralen pharmakokinetischen Barrieren wie Darm, Leber, Niere und Blut Hirn Schranke exprimiert. Das Kit ist konform mit den FDA (2017) und EMA (2013) DDI Leitlinien. Hergestellt von Cell4Pharma.
P-Glykoproteine / MDR1 Assay | 100 Reaktionen
Das Human Pgp Vesicle Kit enthält Inside-out-Membranvesikel aus HEK293-Zellen, die transient P-Glykoprotein (P-gp / MDR1 / ABCB1) überexprimieren. P-gp ist einer der klinisch relevantesten Efflux-Transporter und ist an zentralen pharmakokinetischen Barrieren wie Darm, Leber, Niere und Blut-Hirn-Schranke exprimiert. Das Kit ist konform mit den FDA (2017)- und EMA (2013)-DDI-Leitlinien.
Hergestellt von Cell4Pharma. Eine Variante mit dem neuartigen fluoreszenten FluoPgp-Substrat ist ebenfalls erhältlich (C4PVK011).
Exklusiv im DACH-Raum über SeamlessBio erhältlich.
Transporter-Profil
Name: P-gp, P-Glykoprotein, MDR1, Multidrug Resistance Protein 1, Permeabilitätsglykoprotein, ABCB1, ATP-bindende Kassette Unterfamilie B Mitglied 1
Gen: ABCB1
ATP/AMP-Ratio: >5 (garantiert)
Gewebsexpression: Darm, Leber, Niere, Blut-Hirn-Schranke, viele Tumorzellen
Bekannte Substrate: Convallatoxin, Digoxin, N-Methyl-Chinidin (NMQ), Colchicin, Tacrolimus, Chinidin, Etoposid, Doxorubicin
Lagerbestand: Human-Pgp. Ebenfalls verfügbar: Maus- und Ratten-Pgp
Kit Inhalt (100 Reaktionen)
-
- AMP-ATP Lösung (100 mM), Cell4Pharma
- Assay-Puffer, Cell4Pharma
Hinweis: C4PVK011 enthält zusätzlich das neuartige FluoPgp Fluoreszenzsubstrat für fluoreszenzbasierte Detektion ohne Radiolabeling.
Anwendungen
- P-gp-Substratidentifikation und Efflux-Charakterisierung
- Drug-Drug-Interaction-(DDI)-Studien gemäß FDA/EMA-Leitlinien
- Studien zur Multiresistenz (MDR)-Mechanistik
- In-vitro-Bewertung der Blut-Hirn-Schranken-Penetration
- Inhibitionsstudien und IC50-Bestimmung
- High-Throughput-Screening (Radiolabel-, Fluoreszenz-, LC-MS-Detektion)
Regulatorische Konformität
P-Glykoprotein (ABCB1) ist ein primärer Transporter in den FDA (2017)- und EMA (2013)-DDI-Leitlinien. Die Bewertung des P gp-Substrat-und Inhibitorstatus ist für alle neuen Molekülentitäten (NMEs) mit signifikanter oraler oder systemischer Exposition in der klinischen Entwicklung erforderlich.
Technische Spezifikationen
Wirtszelle: HEK293 (humane embryonale Nierenzelle) Vesikeltyp: Inside-out-Membranvesikel
Format: 100 Reaktionen pro Kit
Detektion: Radiolabel / Fluoreszenz / LC-MS kompatibel Variante mit FluoPgp-Substrat: C4PVK011
Lagerung: –80 °C
Verwandte Produkte
- Human Pgp Vesicle Kit + FluoPgp Substrat (C4PVK011)
- Fluoreszenz-Detektionsvariante, kein Radiolabeling erforderlich
- Human BCRP Vesicle Kit (C4PVK006) – für parallele BCRP-Efflux-Bewertung
- Human Control Vesicle Kit (C4PVK009) – empfohlen als Negativkontrolle
- ciPTEC-OAT1 Assay-Ready Cells – exprimieren funktionelles endogenes P-gp (ABCB1) für zellbasierte Bestätigungsstudien
- Human Serum AB Male OTC (SeamlessBio) – zur Supplementierung von ciPTEC-Kulturmedien
Alle Produkte auf Anfrage.
Kontakt: [email protected] | Telefon: +49 851 37932226
Datasheet
Publikation
Gozalpour E, Wittgen HG, van den Heuvel JJ, et al. Interaction of digitalis-like compounds with p-glycoprotein. Toxicol Sci. 2013,131(2):502-11. doi: 10.1093/toxsci/kfs307
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4.2 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
Surprisingly great for my wide feet and high instep!
Size: 10.5, Color: Velvet Tan/Gum 2
So thrilled I took a chance on these. Finding boots is impossible for me. I have wide feet, a super high instep, a bunion on one foot, and I wear a hard to find size (10.5). I ordered the 10.5 and 11 in these expecting I might need to size up for width (if I could even get them on). It was a struggle getting the 10.5s on the first time, and once I did they were somewhat uncomfortable on the top of my left foot. But I read that they would relax so decided to give it a go because I need waterproof boots for an upcoming trip and the 11s felt too long. After two days of light wear, they are already breaking in and I have no more discomfort. Getting them on is already easier and they are plenty wide enough and very comfortable to walk in. These are true to size. Super happy and worth a day of mild discomfort to break them in!
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Reviewed in the United States on March 7, 2026
★★★★★ 5
Comfortable, sturdy, waterproof
Size: 9.5, Color: Black/Black
Loce, love, love these boots. Have walked ten miles with no foot pain around NYC. They go with everything and and are so comfortable. As they are waterproof/resistant, they get a little warm in high temps, but perfect three-season boots.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Super cute, comfortable, practical, love that they are slip on
Size: 10.5, Color: Tobacco/Black
I have worn these 2-3 times already. The first day was a school/work day so I was on my feet 9 hours and the only issue I had was with the side of the boot rubbing against my ankle. I just make sure I wear socks high enough to cover my ankles or jeans that go into the boots now for long wear days.
Absolutely love them! They keep my feet dry in this snow( we live in Michigan). I had been looking for a pair like this for awhile now and was getting super frustrated that I couldn't find my size in any that I liked. I saw that they had these available in a 10.5 and I wear 11. I looked at the Sorel website reviews (since the amazon ones weren't super helpful on sizing) and they said that the boots ran big so I took a chance on the 10.5 fitting me. They fit perfect. I have long narrow feet but I could see where they would be tight or uncomfortable on someone with wider feet. I've been trying to find a pair for my mom now since she has boot envy.
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Reviewed in the United States on January 27, 2026
★★★★★ 5
Worth it.
Size: 8.5, Color: Crushed Clay/Gum 2
Love all my Sorels. These were really cute, good color, comfortable, warm, fit well, good quality and sturdy. I would order again.
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Reviewed in the United States on March 26, 2026
★★★★★ 5
Fantastic Boots!
Size: 8.5, Color: Redwood/Gum 10
Completely satisfied with these boots. True to size, well made, comfortable from the get-go; not break in needed. Waterproof- really. The Red Suede has withstood a nasty winter season & still looks great. Good traction. I like the ankle height which looks good with long, mid length pants & leggings. I love the easy slip on/off aspect..made getting ready for dog walking easy. I 100%recommend!
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Reviewed in the United States on April 11, 2026